Dissociation of microdissected mouse brain tissue for artifact free single-cell RNA sequencing
2021 | journal article; research paper
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Dissociation of microdissected mouse brain tissue for artifact free single-cell RNA sequencing
Liu, L.; Besson-Girard, S.; Ji, H.; Gehring, K.; Bulut, B.; Kaya, T. & Usifo, F. et al. (2021)
STAR Protocols, 2(2) art. 100590. DOI: https://doi.org/10.1016/j.xpro.2021.100590
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Details
- Authors
- Liu, Lu; Besson-Girard, Simon; Ji, Hao; Gehring, Katrin; Bulut, Buket; Kaya, Tuğberk; Usifo, Fumere; Simons, Mikael ; Gokce, Ozgun
- Abstract
- Single-cell RNA sequencing (scRNA-seq) provides the transcriptome of individual cells and addresses previously intractable problems including the central nervous system's transcriptional responses during health and disease. However, dissociating brain cells is challenging and induces artificial transcriptional responses. Here, we describe an enzymatic dissociation method for mouse brain that prevents dissociation artifacts and lowers technical variations with standardized steps. We tested this protocol on microdissected brain tissue of 3-week- to 24-month-old mice and obtained high-quality scRNA-seq results. For complete details on the use and execution of this protocol, please refer to Safaiyan et al. (2021).
- Issue Date
- 2021
- Journal
- STAR Protocols
- Project
- TRR 274: Checkpoints of Central Nervous System Recovery
TRR 274 | Z02: Genomics and Bioinformatics Platform - Working Group
- RG Gokce (Systems Neuroscience – Cell Diversity)
RG Simons (The Biology of Glia in Development and Disease) - eISSN
- 2666-1667
- Language
- English