A macrophage-T cell coculture model for severe tissue injury-induced T cell death
2021 | journal article; research paper
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Details
- Authors
- Zhu, Jie; Cao, Jiayu; Liesz, Arthur ; Roth, Stefan
- Abstract
- A key observation of tissue injury, such as stroke and burn, is a state of systemic immunosuppression characterized by loss of T cells and rise of infections. Here, we present an in vitro model for cell-cell interactions between innate (macrophages) and adaptive (T cells) immune cells. This protocol facilitates bone marrow-derived macrophages (BMDMs) and splenic T cells in a coculture model. The procedure mimics injury-induced T cell death, which is driven by inflammasome activation in macrophages. For complete details on the use and execution of this protocol, please refer to Roth et al. (2021).
- Issue Date
- 2021
- Journal
- STAR Protocols
- Project
- TRR 274: Checkpoints of Central Nervous System Recovery
TRR 274 | A02: Targeting microglia for the resolution of chronic neuroinflammation after stroke - Working Group
- RG Liesz (Stroke-Immunology)
- eISSN
- 2666-1667
- Language
- English