DNA-PAINT MINFLUX nanoscopy
2022 | journal article. A publication with affiliation to the University of Göttingen.
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- Authors
- Ostersehlt, Lynn M.; Jans, Daniel C. ; Wittek, Anna; Keller-Findeisen, Jan ; Inamdar, Kaushik; Sahl, Steffen J. ; Hell, Stefan W. ; Jakobs, Stefan
- Abstract
- Abstract MINimal fluorescence photon FLUXes (MINFLUX) nanoscopy, providing photon-efficient fluorophore localizations, has brought about three-dimensional resolution at nanometer scales. However, by using an intrinsic on–off switching process for single fluorophore separation, initial MINFLUX implementations have been limited to two color channels. Here we show that MINFLUX can be effectively combined with sequentially multiplexed DNA-based labeling (DNA-PAINT), expanding MINFLUX nanoscopy to multiple molecular targets. Our method is exemplified with three-color recordings of mitochondria in human cells.
- Issue Date
- 2022
- Journal
- Nature Methods
- Project
- EXC 2067: Multiscale Bioimaging
SFB 1190: Transportmaschinen und Kontaktstellen zellulärer Kompartimente
SFB 1190 | P01: Untersuchung der Unterschiede in der Zusammensetzung, Funktion und Position von individuellen MICOS Komplexen in einzelnen Säugerzellen
TRR 274: Checkpoints of Central Nervous System Recovery
TRR 274 | Z01: Bioimaging Platform - Organization
- Max-Planck-Institut für Multidisziplinäre Naturwissenschaften ; Klinik für Neurologie ; Universitätsmedizin Göttingen ; Fraunhofer ITMP - Translationale Neuroinflammation und Automatisierte Mikroskopie TNM
- Working Group
- RG Hell
RG Jakobs (Structure and Dynamics of Mitochondria) - ISSN
- 1548-7091
- eISSN
- 1548-7105
- Language
- English
