Trafficking proteins show limited differences in mobility across different postsynaptic spines

2023-02-17 | journal article

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​Trafficking proteins show limited differences in mobility across different postsynaptic spines​
Mougios, N.; Opazo, F. ; Rizzoli, S. O.   & Reshetniak, S.​ (2023) 
iScience26(2) art. 105971​.​ DOI: https://doi.org/10.1016/j.isci.2023.105971 

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Authors
Mougios, Nikolaos; Opazo, Felipe ; Rizzoli, Silvio O. ; Reshetniak, Sofiia
Abstract
The function of the postsynaptic compartment is based on the presence and activity of postsynaptic receptors, whose dynamics are controlled by numerous scaffolding, signaling and trafficking proteins. Although the receptors and the scaffolding proteins have received substantial attention, the trafficking proteins have not been investigated extensively. Their mobility rates are unknown, and it is unclear how the postsynaptic environment affects their dynamics. To address this, we analyzed several trafficking proteins (α-synuclein, amphiphysin, calmodulin, doc2a, dynamin, and endophilin), estimating their movement rates in the dendritic shaft, as well as in morphologically distinct "mushroom" and "stubby" postsynapse types. The diffusion parameters were surprisingly similar across dendritic compartments, and a few differences between proteins became evident only in the presence of a synapse neck. We conclude that the movement of trafficking proteins is not strongly affected by the postsynaptic compartment, in stark contrast to the presynapse, which regulates strongly the movement of such proteins.
Issue Date
17-February-2023
Journal
iScience 
Project
EXC 2067: Multiscale Bioimaging 
SFB 1190: Transportmaschinen und Kontaktstellen zellulärer Kompartimente 
SFB 1190 | P09: Proteinsortierung in der Synapse: Prinzipien und molekulare Organisation 
SFB 1286: Quantitative Synaptologie 
SFB 1286 | B02: Ein in vitro-Verfahren zum Verständnis der struktur-organisierenden Rolle des Vesikel-Clusters 
Working Group
RG Rizzoli (Quantitative Synaptology in Space and Time) 
RG Opazo (Molecular Probes for Quantitative Neurosciences) 
eISSN
2589-0042
Language
English

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