Modulating the Biomechanical Properties of Engineered Connective Tissues by Chitosan-Coated Multiwall Carbon Nanotubes
2021 | journal article; research paper. A publication with affiliation to the University of Göttingen.
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Modulating the Biomechanical Properties of Engineered Connective Tissues by Chitosan-Coated Multiwall Carbon Nanotubes
Kittana, N.; Assali, M.; Zimmermann, W.-H. ; Liaw, N. ; Santos, G. L. ; Rehman, A. & Lutz, S. (2021)
International Journal of Nanomedicine, Volume 16 pp. 989-1000. DOI: https://doi.org/10.2147/IJN.S289107
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- Authors
- Kittana, Naim; Assali, Mohyeddin; Zimmermann, Wolfram-Hubertus ; Liaw, Norman ; Santos, Gabriela Leao ; Rehman, Abdul; Lutz, Susanne
- Abstract
- Background: Under certain conditions, the physiological repair of connective tissues might fail to restore the original structure and function. Optimized engineered connective tissues (ECTs) with biophysical properties adapted to the target tissue could be used as a substitution therapy. This study aimed to investigate the effect of ECT enforcement by a complex of multiwall carbon nanotubes with chitosan (C-MWCNT) to meet in vivo demands. Materials and Methods: ECTs were constructed from human foreskin fibroblasts (HFF-1) in collagen type I and enriched with the three different percentages 0.025, 0.05 and 0.1% of C-MWCNT. Characterization of the physical properties was performed by biomechanical studies using unidirectional strain. Results: Supplementation with 0.025% C-MWCNT moderately increased the tissue stiffness, reflected by Young’s modulus, compared to tissues without C-MWCNT. Supplementation of ECTs with 0.1% C-MWCNT reduced tissue contraction and increased the elasticity and the extensibility, reflected by the yield point and ultimate strain, respectively. Consequently, the ECTs with 0.1% C-MWCNT showed a higher resilience and toughness as control tissues. Fluorescence tissue imaging demonstrated the longitudinal alignment of all cells independent of the condition. Conclusion: Supplementation with C-MWCNT can enhance the biophysical properties of ECTs, which could be advantageous for applications in connective tissue repair.
- Issue Date
- 2021
- Journal
- International Journal of Nanomedicine
- Project
- SFB 1002: Modulatorische Einheiten bei Herzinsuffizienz
SFB 1002 | C04: Fibroblasten-Kardiomyozyten Interaktion im gesunden und erkrankten Herzen: Mechanismen und therapeutische Interventionen bei Kardiofibroblastopathien
SFB 1002 | S01: In vivo und in vitro Krankheitsmodelle - Working Group
- RG Lutz (G Protein-Coupled Receptor Mediated Signaling)
RG Zimmermann (Engineered Human Myocardium) - eISSN
- 1178-2013
- Language
- English