Spatiotemporal resolution of Ca2+ signaling events by real time imaging of single B cells
2012 | journal article. A publication with affiliation to the University of Göttingen.
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Details
- Authors
- Junek, Stephan; Engelke, Michael; Schild, Detlev; Wienands, Juergen
- Abstract
- Antigen-induced B cell activation requires mobilization of the Ca2+ second messenger. This process is associated with the subcellular relocalization of signal effector proteins of the B cell antigen receptor such as the adaptor protein SLP65. Here we describe a broadly applicable live cell imaging method to simultaneously visualize intracellular Ca2+ flux profiles and the translocation of cytosolic signaling proteins to the plasma membrane in real time. Our approach delineated the kinetic hierarchy of Ca2+ signaling events in B cells and revealed a timely ordered contribution of various organelles to the overall Ca2+ signal. The developed experimental setup provides a useful tool to resolve the spatiotemporal signaling dynamics in various receptor signaling systems. (c) 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
- Issue Date
- 2012
- Status
- published
- Publisher
- Elsevier Science Bv
- Journal
- FEBS Letters
- ISSN
- 0014-5793