Improved fluorescent (calcium indicator) dye uptake in brain slices by blocking multidrug resistance transporters

2008 | journal article. A publication with affiliation to the University of Göttingen.

Jump to: Cite & Linked | Documents & Media | Details | Version history

Cite this publication

​Improved fluorescent (calcium indicator) dye uptake in brain slices by blocking multidrug resistance transporters​
Manzini, I.; Schweer, T.-S. & Schild, D. ​ (2008) 
Journal of Neuroscience Methods167(2) pp. 140​-147​.​ DOI: https://doi.org/10.1016/j.jneumeth.2007.07.018 

Documents & Media

Artikel1.07 MBAdobe PDF

License

Published Version

Special user license Goescholar License

Details

Authors
Manzini, Ivan; Schweer, Tina-Saskia; Schild, Detlev 
Abstract
ATP-binding cassette (ABC) transporters are a family of transmembrane proteins that, also known as multidrug resistance proteins, transport a wide variety of substrates across biological membranes in an energy-dependent manner. Recently it has been shown that members of this protein family interfere with fluorescent (calcium indicator) dye uptake in taste buds of rat and in cells in the olfactory epithelium of larval Xenopus laevis, including olfactory receptor neurons. It has, however, not been resolved whether this effect only serves to extrude xenobiotics in sensory taste and olfactory cells, or alternatively, whether it is a more general feature of many central nervous system neurons. In the latter case blocking these transporters would improve fluorescent dye uptake in general. Here we show, by means of cell imaging. that also neurons of the olfactory bulb express multidrug resistance transporters, whereby a marked inhomogeneity among cells in the main and accessory olfactory bulb was observed. Blocking these transporters improved the net uptake of fluorescent dyes not only in cell somata of the olfactory bulb, but especially in fine neuronal structures such as individual dendrites or olfactory glomeruli, which consist of a tangle of tiny neuronal processes. We therefore suggest that the expression of multidrug resistance proteins may be common in cells of the central nervous system, and that the application of specific transport inhibitors could generally improve fluorescent dye uptake in brain slices, thereby improving calcium imaging conditions. (c) 2007 Elsevier B.V. All rights reserved.
Issue Date
2008
Journal
Journal of Neuroscience Methods 
ISSN
0165-0270
Language
English

Reference

Citations


Social Media