Fluorescence correlation spectroscopy in small cytosolic compartments depends critically on the diffusion model used

2000-12-01 | journal article. A publication with affiliation to the University of Göttingen.

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​Fluorescence correlation spectroscopy in small cytosolic compartments depends critically on the diffusion model used​
Gennerich, A. & Schild, D.​ (2000) 
Biophysical journal79(6) pp. 3294​-3306​.​ DOI: https://doi.org/10.1016/S0006-3495(00)76561-1 

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Authors
Gennerich, A.; Schild, D.
Abstract
Fluorescence correlation spectroscopy (FCS) is a powerful technique for measuring low concentrations of fluorescent molecules and their diffusion constants. In the standard case, fluorescence fluctuations are measured in an open detection volume defined by the confocal optics. However, if FCS measurements are carried out in cellular processes that confine the detection volume, the standard FCS model leads to erroneous results. In this paper, we derive a modified FCS model that takes into account the confinement of the detection volume. Using this model, we have carried out the first FCS measurements in dendrites of cultured neurons. We further derive, for the case of confined diffusion, the limits within which the standard two- and three-dimensional diffusion models give reliable results.
Issue Date
1-December-2000
Status
published
Publisher
Biophysical Society
Journal
Biophysical journal 
Organization
Universitätsmedizin Göttingen
ISSN
0006-3495
Language
English

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