A potassium channel protein encoded by chlorella virus PBCV-1
2000 | journal article. A publication with affiliation to the University of Göttingen.
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A potassium channel protein encoded by chlorella virus PBCV-1
Plugge, B.; Gazzarrini, S.; Nelson, M.; Cerana, R.; van Etten, J. L.; Derst, C. & DiFrancesco, D. et al. (2000)
Science, 287(5458) pp. 1641-1644. DOI: https://doi.org/10.1126/science.287.5458.1641
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Details
- Authors
- Plugge, B.; Gazzarrini, S.; Nelson, M.; Cerana, R.; van Etten, J. L.; Derst, C.; DiFrancesco, D.; Moroni, A.; Thiel, G.
- Abstract
- The Large chlorella virus PBCV-1, which contains double-stranded DNA (dsDNA), encodes a 94-codon open reading frame (ORF) that contains a motif resembling the signature sequence of the pore domain of potassium channel proteins. Phylogenetic analyses of the encoded protein, Kcv, indicate a previously unidentified type of potassium channel. The messenger RNA encoded by the ORF Leads to functional expression of a potassium-selective conductance in Xenopus laevis oocytes. The channel blockers amantadine and barium, but not cesium, inhibit this conductance, in addition to virus plaque formation. Thus, PBCV-1 encodes the first known viral protein that functions as a potassium-selective channel and is essential in the virus Life cycle.
- Issue Date
- 2000
- Status
- published
- Publisher
- Amer Assoc Advancement Science
- Journal
- Science
- ISSN
- 0036-8075
- Sponsor
- NIGMS NIH HHS [GM32441, GM41333]; Telethon [971]
