AP-4 binds basolateral signals and participates in basolateral sorting in epithelial MDCK cells
2002 | journal article. A publication with affiliation to the University of Göttingen.
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AP-4 binds basolateral signals and participates in basolateral sorting in epithelial MDCK cells
Simmen, T.; Honing, S.; Icking, A.; Tikkanen, R. & Hunziker, W. (2002)
Nature Cell Biology, 4(2) pp. 154-159. DOI: https://doi.org/10.1038/ncb745
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- Authors
- Simmen, T.; Honing, S.; Icking, A.; Tikkanen, R.; Hunziker, W.
- Abstract
- Adaptors are heterotetrameric complexes that mediate the incorporation of cargo into transport vesicles by interacting with sorting signals present in the cytosolic domain of transmembrane proteins. Four adaptors, AP-1 (beta1, gamma, mu1A or mu1B, sigma1), AP-2 (beta2, alpha, mu2, sigma2), AP-3 (beta3, delta, mu3, sigma3) or AP-4 (beta4, epsilonc, mu4, sigma4), have been characterized(1,2). AP1 and AP-3 mediate sorting events at the level of the TGN and/or endosomes, whereas AP-2 functions in endocytic clathrin coated vesicle formation; no function is known so far for AP-4. Here, we show that AP-4 can bind different types of cytosolic signals known to mediate basolateral transport in epithelial cells. Furthermore, in MDCK cells with depleted mu4 protein levels, several basolateral proteins are mis-sorted to the apical surface, showing that AP-4 participates in basolateral sorting in epithelial cells.
- Issue Date
- 2002
- Status
- published
- Publisher
- Nature Publishing Group
- Journal
- Nature Cell Biology
- ISSN
- 1465-7392
- Sponsor
- Fondazione Telethon