Single- and two-photon imaging of human micrometastases and disseminated tumour cells with conjugates of nanobodies and quantum dots.
2018 | journal article. A publication with affiliation to the University of Göttingen.
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Single- and two-photon imaging of human micrometastases and disseminated tumour cells with conjugates of nanobodies and quantum dots.
Ramos-Gomes, F.; Bode, J.; Sukhanova, A.; Bozrova, S. V.; Saccomano, M.; Mitkovski, M. & Krueger, J. E. et al. (2018)
Scientific Reports, 8(1) art. 4595. DOI: https://doi.org/10.1038/s41598-018-22973-8
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Details
- Authors
- Ramos-Gomes, Fernanda; Bode, Julia; Sukhanova, Alyona; Bozrova, Svetlana V.; Saccomano, Mara; Mitkovski, Miso; Krueger, Julia Eva; Wege, Anja K.; Stuehmer, Walter; Samokhvalov, Pavel S.; Baty, Daniel; Chames, Patrick; Nabiev, Igor; Alves, Frauke
- Abstract
- Early detection of malignant tumours and, especially, micrometastases and disseminated tumour cells is still a challenge. In order to implement highly sensitive diagnostic tools we demonstrate the use of nanoprobes engineered from nanobodies (single-domain antibodies, sdAbs) and fluorescent quantum dots (QDs) for single- and two-photon detection and imaging of human micrometastases and disseminated tumour cells in ex vivo biological samples of breast and pancreatic metastatic tumour mouse models expressing human epidermal growth factor receptor 2 (HER2) or carcinoembryonic antigen (CEA). By staining thin (5-10 µm) paraffin and thick (50 µm) agarose tissue sections, we detected HER2- and CEA-positive human tumour cells infiltrating the surrounding tissues or metastasizing to different organs, including the brain, testis, lung, liver, and lymph nodes. Compared to conventional fluorescently labelled antibodies the sdAb-HER2-QD and sdAb-CEA-QD nanoprobes are superior in detecting micrometastases in tissue sections by lower photobleaching and higher brightness of fluorescence signals ensuring much better discrimination of positive signals versus background. Very high two-photon absorption cross-sections of QDs and small size of the nanoprobes ensure efficient imaging of thick tissue sections unattainable with conventional fluorescent probes. The nanobody-QD probes will help to improve early cancer diagnosis and prognosis of progression by assessing metastasis.
- Issue Date
- 2018
- Journal
- Scientific Reports
- Project
- info:eu-repo/grantAgreement/EC/FP7/246479/EU//NAMDIATREAM
- Organization
- Max-Planck-Institut für Experimentelle Medizin ; Klinik für Hämatologie und Medizinische Onkologie
- ISSN
- 2045-2322
- Language
- English