Nanoscopic anatomy of dynamic multi-protein complexes at membranes resolved by graphene-induced energy transfer
2021 | journal article; research paper. A publication with affiliation to the University of Göttingen.
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Nanoscopic anatomy of dynamic multi-protein complexes at membranes resolved by graphene-induced energy transfer
Füllbrunn, N.; Li, Z.; Jorde, L.; Richter, C. P; Kurre, R.; Langemeyer, L. & Yu, C. et al. (2021)
eLife, 10. DOI: https://doi.org/10.7554/eLife.62501
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Details
- Authors
- Füllbrunn, Nadia; Li, Zehao; Jorde, Lara; Richter, Christian P; Kurre, Rainer; Langemeyer, Lars; Yu, Changyuan; Meyer, Carola; Enderlein, Jörg ; Ungermann, Christian; Piehler, Jacob; You, Changjiang
- Abstract
- Insights into the conformational organization and dynamics of proteins complexes at membranes is essential for our mechanistic understanding of numerous key biological processes. Here, we introduce graphene-induced energy transfer (GIET) to probe axial orientation of arrested macromolecules at lipid monolayers. Based on a calibrated distance-dependent efficiency within a dynamic range of 25 nm, we analyzed the conformational organization of proteins and complexes involved in tethering and fusion at the lysosome-like yeast vacuole. We observed that the membrane-anchored Rab7-like GTPase Ypt7 shows conformational reorganization upon interactions with effector proteins. Ensemble and time-resolved single-molecule GIET experiments revealed that the HOPS tethering complex, when recruited via Ypt7 to membranes, is dynamically alternating between a ‘closed’ and an ‘open’ conformation, with the latter possibly interacting with incoming vesicles. Our work highlights GIET as a unique spectroscopic ruler to reveal the axial orientation and dynamics of macromolecular complexes at biological membranes with sub-nanometer resolution.
- Issue Date
- 2021
- Journal
- eLife
- Project
- EXC 2067: Multiscale Bioimaging
- Working Group
- RG Enderlein
- eISSN
- 2050-084X
- Language
- English